The CACHE challenge was featured in a recent webinar by the Target 2035 initative. Watch the recording and learn more about the CACHE challenge.
In addtion, see the CACHE Roadmap article on Nature Reviews Chemistry, to learn more on CACHE.
All compounds will be tested at the CACHE experimental hub, Structural Genomics Consortium (SGC), University of Toronto. The affinity of compounds that are active in a primary screen, typically in a surface plasmon resonance (SPR) assay, will be evaluated with an orthogonal method. The solubility and colloidal aggregation of hit molecules will be determined experimentally by dynamic light scattering. Common pan-assay interference (PAINS) compounds will be flagged. The lab is renowned for its high-quality chemical probes for proteins such as NDS2, PRDM9, PRMT7, NSD3, EED, SUV420 or WDR5.
CACHE is modelled after CASP, the community wide experiment on developing computational methods to predict protein structure from primary sequence and the virtual screening competition D3R.
CACHE is a component of the Target 2035 initiative which is an open science global movement consisting of international scientists and researchers, focusing on the creation of chemical and biological tools to study human proteins and inform drug discovery.